- Publication date : 1990-06-26
Leclerc P, Sirard MA, Chafouleas JG, Lambert RD. Decreased binding of calmodulin to bull sperm proteins during heparin-induced capacitation. Biol. Reprod. 1990;42:483-9. PubMed PMID: 2340333.
animals calmodulin calmodulin-binding proteins carrier proteins cattle dose-response relationship, drug fertilization heparin male seminal plasma proteins sperm capacitation
The 125I-calmodulin gel overlay procedure was used to evaluate the effect of a heparin treatment on the calmodulin-binding proteins of bull spermatozoa. At concentrations that increase the in vitro fertilization rate of in vitro-matured oocytes, heparin induced a decrease in the binding to calmodulin (CaM) in 3 sperm proteins of 28, 30, and 49 kDa. The binding of these proteins to CaM was higher when Ca2+ was absent from the overlay procedure, and this binding was negatively correlated to the fertilization rate. These results suggest that sperm capacitation is associated with a decrease in the binding of CaM to the 28, 30, and 49 kDa sperm CaM-binding proteins. Implications of such a decrease are discussed.