- Publication date : 2016-11-09
Grégoire MC, Massonneau J, Leduc F, Arguin M, Brazeau MA, Boissonneault G. Quantification and genome-wide mapping of DNA double-strand breaks. DNA Repair (Amst.). 2016;48:63-68. doi: 10.1016/j.dnarep.2016.10.005. PubMed PMID: 27825743.
chromosome mapping dna dna breaks, double-stranded dna nucleotidylexotransferase dna repair deoxyribonucleases, type ii site-specific genetic loci genome, human hela cells humans kinetics phosphorus radioisotopes plasmids saccharomyces cerevisiae proteins transfection
DNA double-strand breaks (DSBs) represent a major threat to the genetic integrity of the cell. Knowing both their genome-wide distribution and number is important for a better assessment of genotoxicity at a molecular level. Available methods may have underestimated the extent of DSBs as they are based on markers specific to those undergoing active repair or may not be adapted for the large diversity of naturally occurring DNA ends. We have established conditions for an efficient first step of DNA nick and gap repair (NGR) allowing specific determination of DSBs by end labeling with terminal transferase. We used DNA extracted from HeLa cells harboring an I-SceI cassette to induce a targeted nick or DSB and demonstrated by immunocapture of 3'-OH that a prior step of NGR allows specific determination of loci-specific or genome wide DSBs. This method can be applied to the global determination of DSBs using radioactive end labeling and can find several applications aimed at understanding the distribution and kinetics of DSBs formation and repair.