- Publication date : 2010-04-16
Delbès G, Vaisheva F, Luu T, Marcon L, Hales BF, Robaire B. Reversibility of the effects of the chemotherapeutic regimen for non-Hodgkin lymphoma, cyclophosphamide, doxorubicin, vincristine, and prednisone, on the male rat reproductive system and progeny outcome. Reprod. Toxicol. 2010;29:332-8. doi: 10.1016/j.reprotox.2010.01.007. PubMed PMID: 20096776.
animals antineoplastic agents antineoplastic combined chemotherapy protocols clinical protocols cyclophosphamide doxorubicin epididymis female humans litter size lymphoma, non-hodgkin male prednisone random allocation rats rats, sprague-dawley Reproduction sperm count spermatozoa testis treatment outcome vincristine
The chemotherapeutic agents used to treat non-Hodgkin lymphoma, cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP), have adverse effects on male reproductive function and progeny outcome. To determine the reversibility of these effects, male rats received a CHOP treatment mimicking human exposure. CHOP reduced testicular and epididymal weights; these remained decreased after 9 weeks recovery. This treatment also decreased testicular sperm number and increased spermatozoal DNA damage. Although sperm production returned to control values after 9 weeks recovery, DNA damage persisted. Decreased litter size, and increased pre- and post-implantation losses were observed among litters sired by CHOP-exposed males. Litter size and pre-implantation loss returned to control within 3 weeks post-treatment and post-implantation loss by 6 weeks. Thus, effects of CHOP on progeny outcome were reversed 9 weeks post-treatment, although germ cell DNA breaks remained elevated. These data suggest that the ability to sire viable progeny may not be a sensitive measure of spermatozoal quality in rats.