Robert, Claude PhD - Regular researcher


Full professor - Université Laval

Department of animal science,

Institute of nutrition and functional foods,

Faculty of agriculture and food science

CRDSI Co-director 

Research interest

My research program is composed of two main axes. The first one begin the definition of oocyte and early embryo quality determinants. The fact that the ability of the oocyte to become an embryo is acquired when the gamete is still in the ovarian follicle is well known. The ovarian follicle is composed of about four cellular types and we are interested in the cells closer to the oocyte by studying their ability to sustain the development and preparation of the gamete. We are also interested in the RNA pools stored in the oocyte and used to sustain the first cellular divisions. To this day, the storing, management and disposal mechanism for these RNA pools are not well understood. This despite the fact that the majority of embryos die during the first cellular divisions while these stocks support cellular functions.

Our second research axis revolves around genetic related aspects such as genomics (studying DNA as a whole), including genomic diversity analysis and the impact of epigenetic, especially DNA methylation, on gene expression and thus phenotypes. Representative examples of our projects in this axis are the analysis of the genetic diversity of the Canadienne cow breed or the impact of the environment (such as the one being affected by the mother’s nutrition) on embryo quality and the establishment of its epigenome; more precisely on the DNA methylation marks that will influence gene expression, including a range of long non-coding RNA.    

Expertise

Early embryonic development Genetics Genomics Maternal RNA management Oocyte quality

INAF/ Pavillon des Services

2440 boul Hochelaga
Université Laval
Québec (QC) Canada
G1V 0A6
(418) 656-2131 extension : 12842
Fax : (418) 656-5877
claude.robert@fsaa.ulaval.ca

Postoctoral fellowship: Biomedical Sciences, University of Guelph (Guelph, Ontario, Canada)

PhD: Sciences Animales, Université Laval (Québec, Québec, Canada)

MSc: Sciences Animales, Université Laval (Québec, Québec, Canada)

Undergraduate: Biochimie, Université de Sherbrooke (Sherbrooke, Québec, Canada)


Direction
Picard, Clémence - Master - Studies in progress
Subject :

Études combinées de cohortes et d'intervention pour l'évaluation inter-espèce des facteurs métaboliques communs reliés à l'infertilité et son traitement par des lipides fonctionnels

Dubuc, Karine - Master - Studies in progress
Subject :

Étude de l’établissement du réseau de communication entre l’ovocyte et le cumulus  

Nenonene, Karen - Master - Studies in progress
Subject :

Détermination du rôle de la protéine du X-fragile (FMRP) dans l’ovaire  



codirection
Lafontaine, Simon - Master - Studies in progress
Subject :

Analyse des gènes à empreinte d'embryons bovins issus des techniques de reproduction assistée  

Direction
Carrier, Alexandra - PhD - Studies in progress
Subject :

Amélioration des caractères de santé et fertilité chez la vache laitière par une approche génomique alternative



codirection
Fortin, Chloé - PhD - Studies in progress
Subject :

Transcriptomic signature of ovarian follicles associated with failed in vitro fertilization attempts in women. 

Chen, Hong - PhD - Studies in progress
Subject :

Étude du profil épigénétique spermatique humain au cours de la maturation post-testiculaire


5 Latest projects

  • Fragile X Mental retardation1 gene: a master switch of ovarian communication and oocyte quality
  • Chips for better chops: Commercial application of genomics for accelerated swine genetic improvement
  • Improvement of health and fertility characteristics in dairy cows using an alternative genomic approach
  • Cross-species combination of cohort and intervention studies to assess common metabolic factors related to infertility and the use of functional lipids for its treatment
  • Description of the genetic populations of the Canadienne cow: essential step for the develpment of a breed management strategy

5 Latest projects

  • La protéine spermatique SPAM1 comme marqueur de la qualité de la semence, de la fertilité des taureaux, et de la vocation laitière ou bouchère du cheptel bovin québécois
  • Analyse ciblée de la méthylation d'ADN dans la lignée germinale mâle pour le dépistage de l'influence environnementale
  • Impact of the sperm epigenome on the progeny outcome born by assisted reproductive technology
  • Perfect follicles for perfect oocytes
  • Epigenetic and transcriptomic analysis of embryos from oocytes or sperm from peri-pubertal bovine