Sirard, Marc-André DMV, PhD - Regular researcher


Full professor - Université Laval

Department of animal science,

Institute of nutrition and functional foods,

Faculty of agriculture and food science

Research interest

Journeay to the center of the follicle

The main focus of our research is oocyte competency, which essentially depends on its accompanying follicle. Our genomic tools enable the discovery and study of markers for good follicles in both bovine and human in order to improve procedures, especially in vitro fertilization.

It appears that the mammalian ovary can determine whether the oocyte will be a good one. It now also appears that endogenous and exogenous (injection) hormones can affect follicular quality and incidentally that of the oocyte. The same would be true of environmental conditions such as the mother’s nutrition or her metabolic status. We are interested in understanding who genes react to these conditions and how the ovary can then modify the epigenomic signature of the oocyte to transmit this information.

 

Our studies are follow up by transcriptome (the set of genes express in a tissue) and epigenome (the packaging of each gene in a tissue) analyses in early bovine embryos using the new EmbryoGENE platform. These analyses enable us to measure the impact of all of the possible manipulation on an embryo, from simple culture to cloning or transgenesis. 

Expertise

Granulosa cells Cumulus cells Oocyte competence Competence markers Transcriptomics

INAF/ Pavillon des Services

2440 boul Hochelaga
Université Laval
Québec (Québec), G1V 0A6
(418) 656-2131 extension : 7359
Fax : (418) 656-5877
marc-andre.sirard@fsaa.ulaval.ca

Postdoctoral fellowship: Animales Sciences, Wisconsin University (Madison, WI, USA)

PhD: Médecine expérimentale, Université Laval (Québec, QC, Canada)

Veterinary medicine: École de médecine vétérinaire, Université de Montréal (Saint-Hyacinthe, QC, Canada)


Professionnels de recherche
Stagiaires

Direction
Chaput, Catherine - Master - Studies in progress
Lafontaine, Simon - Master - Studies in progress
Subject :

Analyse des gènes à empreinte d'embryons bovins issus des techniques de reproduction assistée  



Direction
Shi, Meihong - PhD - Studies in progress
Tremblay, Patricia - PhD - Studies in progress
Subject :

Caractérisation des voies de signalisation PKA et PKC dans les cellules de la granulosa humain en culture

Fortin, Chloé - PhD - Studies in progress
Subject :

Transcriptomic signature of ovarian follicles associated with failed in vitro fertilization attempts in women. 

Wu, Chongyang - PhD - Studies in progress
Subject :

The effect of bulls age on sperm methylation, small RNAs and embryo gene expression

Zhang, Ying - PhD - Studies in progress
Subject :

DNA methylation biomarkers of fertility in bulls  




5 Latest projects

  • Demonstration of the biological and economic effects of postponing from day 60 to day 120 post-partum the insemination of cows suffering from metabolic/nutritional stress
  • Perfect follicles for perfect oocytes
  • National research strategy on inter-generational epigenetic in reproduction
  • Cost2: control Ovarian stimulation timing test
  • Programming the oocyte for the next generation