Sirard, Marc-André DMV, PhD - Regular researcher


Full professor - Université Laval

Department of animal science,

Institute of nutrition and functional foods,

Faculty of agriculture and food science

Research interest

Journeay to the center of the follicle

The main focus of our research is oocyte competency, which essentially depends on its accompanying follicle. Our genomic tools enable the discovery and study of markers for good follicles in both bovine and human in order to improve procedures, especially in vitro fertilization.

It appears that the mammalian ovary can determine whether the oocyte will be a good one. It now also appears that endogenous and exogenous (injection) hormones can affect follicular quality and incidentally that of the oocyte. The same would be true of environmental conditions such as the mother’s nutrition or her metabolic status. We are interested in understanding who genes react to these conditions and how the ovary can then modify the epigenomic signature of the oocyte to transmit this information.

 

Our studies are follow up by transcriptome (the set of genes express in a tissue) and epigenome (the packaging of each gene in a tissue) analyses in early bovine embryos using the new EmbryoGENE platform. These analyses enable us to measure the impact of all of the possible manipulation on an embryo, from simple culture to cloning or transgenesis. 

Expertise

Granulosa cells Cumulus cells Oocyte competence Competence markers Transcriptomics Embryo Epigenetics Genomics Ovary Ovule

INAF/ Pavillon des Services

2440 boul Hochelaga, Local 2732
Université Laval
Québec (Québec), G1V 0A6
(418) 656-2131 extension : 407359
Fax : (418) 656-5877
marc-andre.sirard@fsaa.ulaval.ca

Postdoctoral fellowship: Animales Sciences, Wisconsin University (Madison, WI, USA)

PhD: Médecine expérimentale, Université Laval (Québec, QC, Canada)

Veterinary medicine: École de médecine vétérinaire, Université de Montréal (Saint-Hyacinthe, QC, Canada)


Professionnels de recherche

Direction
Chaput, Catherine - Master - Studies in progress


Direction
Shi, Meihong - PhD - Studies in progress
Tremblay, Patricia - PhD - Studies in progress
Subject :

Caractérisation des voies de signalisation PKA et PKC dans les cellules de la granulosa humain en culture

Fortin, Chloé - PhD - Studies in progress
Subject :

Transcriptomic signature of ovarian follicles associated with failed in vitro fertilization attempts in women. 

Wu, Chongyang - PhD - Studies in progress
Subject :

The effect of bulls age on sperm methylation, small RNAs and embryo gene expression

Zhang, Ying - PhD - Studies in progress
Subject :

Epigenetic biomarkers screening of bull fertility

Lafontaine, Simon - PhD - Studies in progress
Subject :

Comparaison de vaches issues de fécondation in vitro et d'insémination artificielle pour les critères de santé, fertilité et productivité

Arjoune, Asma - PhD - Studies in progress
Subject :

Effet de la mélatonine sur l'axe reproductif chez les ruminants



Direction
de Lima, Camila Bruna - Postdoctoral fellow - Studies in progress
Subject :

Analysis of mitochondrial methylation patterns in oocytes and embryos using the bovine model
 




5 Latest projects

  • Projet de développement d’un outil d’analyse de la méthylation de l’ADN sur le génome mitochondrial à l’aide du nano-séquençages sur plateforme Oxford nanopores
  • Comparaison des vaches issues de fécondation in vitro et de l'insémination artificielle sur les critères de fertilité, santé et productivité
  • Integrating genomic aproaches to improve dairy cattle resilience : A comprehensive goal to enhance Canadian dairy industry sustainnability
  • DNA methylation biomarkers of fertility in bulls
  • Traitement ciblé de l'infertilité post-partum chez la vache laitière

2 Latest projects

  • Améliorer la rentabilité des fermes par une sélection plus hâtive et une meilleure gestion des veaux
  • Observatoire international sur les impacts sociétaux de l'intelligence artificielle et du numérique