- Date de publication : 2003-01-06
Fournier V, Leclerc P, Cormier N, Bailey JL. Implication of calmodulin-dependent phosphodiesterase type 1 during bovine sperm capacitation. J. Androl. 2003 Jan-Feb;24:104-12. PubMed PMID: 12514091.
3',5'-cyclic-amp phosphodiesterases aniline compounds animals anti-bacterial agents cattle chlortetracycline cyclic nucleotide phosphodiesterases, type 1 eosine yellowish-(ys) female fertilization in vitro fluorescent dyes isoenzymes male sperm capacitation staining and labeling
Phosphodiesterases (PDEs) are enzymes that degrade cyclic nucleotides. The calcium-calmodulin dependent PDE type 1 (PDE 1) and the cyclic adenosine monophosphate (cAMP)-specific PDE type 4 (PDE 4) have been implicated in sperm function. We tested the hypothesis that specific PDEs regulate capacitation of bovine sperm in a manner independent of those that mediate motility. Our objectives were to determine the effects of inhibiting PDE 1 and PDE 4 on capacitation and motility, and to compare these effects to those of heparin, which is necessary for capacitation of bull sperm in vitro. Fresh sperm were supplemented either with 15 microg/mL heparin (positive control) or the PDE inhibitors vinpocetine (specific for PDE 1) and rolipram (specific for PDE 4), and then incubated for 5 hours. At 0, 3, and 5 hours, samples were assayed for capacitation and motility parameters according to the chlortetracycline (CTC) fluorescent pattern B and computer-assisted sperm analysis, respectively. A higher percentage of CTC pattern B sperm relative to heparin controls was observed at 0 and 3 hours when sperm were incubated with vinpocetine. After 5 hours, the percentage of heparin- and vinpocetine-treated sperm showing pattern B did not differ (P >.05). Rolipram did not affect CTC patterns (P >.05; n = 4). Vinpocetine and heparin both reduced the percentage of progressively motile sperm after 3 and 5 hours, but vinpocetine reduced it more than heparin (P <.05; n = 4). Rolipram transiently increased linearity versus sperm with heparin (P <.05; n = 4). To further test the hypothesis that PDE 1 inhibition permits capacitation, we conducted in vitro fertilization. Vinpocetine did not support the ability of sperm to penetrate homologous oocytes (n = 5). Although cAMP regulation by PDE 1 may occur early during capacitation, downstream events appear to prevent full capacitation from occurring prematurely.