- Date de publication : 2010-10-12
Sylvestre EL, Pennetier S, Bureau M, Robert C, Sirard MA. Investigating the potential of genes preferentially expressed in oocyte to induce chromatin remodeling in somatic cells. Cell Reprogram. 2010;12:519-28. doi: 10.1089/cell.2010.0012. PubMed PMID: 20936903.
base sequence cell dedifferentiation cell line cell proliferation chromatin assembly and disassembly dna primers epigenesis, genetic female gene expression regulation, developmental homeodomain proteins humans octamer transcription factor-3 oocytes pluripotent stem cells rna, messenger soxb1 transcription factors transfection
The oocyte capacity to rejuvenate a differentiated nucleus to restart the proper embryonic program has been highly conserved between vertebrate species. In view of the recent progress to induce pluripotency in somatic cells with stemness genes, we investigated the potential of oocyte genes to contribute to chromatin rearrangements in somatic cells. We selected conserved genes that are naturally expressed mainly in oocytes and that were susceptible to play a role in reprogramming during early embryogenesis. We induced their expression by transient transfection in HEK293 cells. We then assessed whether they had a global impact on epigenetic events such as histone core modifications, and also on transcription and expression of pluripotency-associated transcription factors. Nucleoplasmin 2 (NPM2), activation-induced cytidine deaminase (AICDA), and Geminin (GMNN) overexpression induced differences in histone core modifications (methylation and acetylation). AICDA and NPM2 also influenced RNA neosynthesis. NPM2, GMNN, and STELLA induced overexpression of well-known pluripotency transcription factors. Overall, AICDA, GMNN, NPM2, and STELLA influenced at least one of the aspects analyzed. Their potential could be useful in increasing the cell receptivity to pluripotency induction.