- Date de publication : 2012-04-02
Delbes G, Yanagiya A, Sonenberg N, Robaire B. PABP interacting protein 2A (PAIP2A) regulates specific key proteins during spermiogenesis in the mouse. Biol. Reprod. 2012;86:95. doi: 10.1095/biolreprod.111.092619. PubMed PMID: 22190698.
a kinase anchor proteins animals carrier proteins eukaryotic initiation factor-4g fertility flagella gene expression profiling hexokinase male mice mice, knockout models, animal proteomics sperm motility spermatogenesis tumor suppressor proteins
During spermiogenesis, expression of the specific proteins needed for proper differentiation of male germ cells is under translational control. We have shown that PAIP2A is a major translational regulator involved in the maturation of male germ cells and male fertility. To identify the proteins controlled by PAIP2A during spermiogenesis, we characterized the proteomic profiles of elongated spermatids from wild-type (WT) mice and mice that were Paip2a/Paip2b double-null mutants (DKO). Elongated spermatid populations were obtained and proteins were extracted and separated on gradient polyacrylamide gels. The gels were digested with trypsin and peptides were identified by mass spectrometry. We identified 632 proteins with at least two unique peptides and a confidence level of 95%. Only 209 proteins were consistently detected in WT or DKO replicates with more than five spectra. Twenty-nine proteins were differentially expressed with at least a 1.5-fold change; 10 and 19 proteins were down- and up-regulated, respectively, in DKO compared to WT mice. We confirmed the significantly different expression levels of three proteins, EIF4G1, AKAP4, and HK1, by Western blot analysis. We have characterized novel proteins that have their expression controlled by PAIP2A; of these, 50% are involved in flagellar structure and sperm motility. Although several proteins affected by abrogation of Paip2a have established roles in reproduction, the roles of many others remain to be determined.